The full plot follows Bond from a murder inquiry in Jamaica to the destruction of a villain's island base. After Strangways and his secretary are murdered, M sends James Bond to investigate. Bond meets Felix Leiter of the CIA and the local fisherman Quarrel, who becomes his ally.
Bond's inquiries lead him to Professor Dent, a geologist working for the mysterious Dr. No, and he ends up killing Dent. He also has the double agent Miss Taro arrested. Bond and Quarrel then travel to Crab Key, the island where the scientist is based, and meet Honey Ryder, a shell diver. A flame throwing vehicle kills Quarrel, and Bond and Honey are captured.
The villain, a SPECTRE member with prosthetic metal hands, tries to persuade Bond to join him. Bond refuses and is held prisoner. He escapes and sabotages the base's nuclear reactor while a rocket launch is being disrupted. The doctor dies in the reactor pool. Bond rescues Honey, and the two flee the island by boat, where Felix Leiter finds them and picks them up.
In four stages, Bond investigates the murders in Jamaica, follows the trail through Dent and Miss Taro, reaches Crab Key and is captured, and then escapes to wreck the base from the inside.